Suppression of human monocyte tissue factor induction by red wine phenolics and synthetic derivatives of resveratrol

forrás: http://www.ncbi.nlm.nih.gov/pubmed/16507316


2014-10-28 13:42:48


Prevention of cardiovascular disease through nutritional supplements is growing in popularity throughout the world. Multiple epidemiologic studies found that moderate consumption of alcohol, particularly red wine, lowers mortality rates from coronary heart diseases (CHD). Chronic inflammation and atherosclerosis associated with CHD culminate in aberrant intravascular expression of tissue factor (TF), which triggers blood coagulation leading to thrombosis, a major cause for heart attack. We showed earlier that two red wine phenolics, resveratrol and quercetin, suppressed TF induction in endothelial cells. In the present study, we investigated efficacy of seven resveratrol derivatives, which were shown to be effective in regulating cancer cell growth in vitro at much lower concentrations than the parent compound resveratrol, in inhibiting TF induction in peripheral blood mononuclear cells (PBMCs). We also tested possible synergistic effects of resveratrol and quercetin with the other major red wine phenolics in suppression of lipopolysaccharide-induced TF expression in human PBMCs. We found that several resveratrol derivatives were 2- to 10-fold more efficient than resveratrol in inhibiting TF induction. Our study found no evidence for synergism among red wine polyphenolics. These data suggest that structural alterations of resveratrol can be effective in producing potent antithrombotic agents that will have therapeutic potential in the improvement of cardiovascular health and prevention of CHD. Among major red wine phenolics, quercetin appears to be the predominant suppressor of TF induction.


Resveratrol induces gastric cancer cell apoptosis via reactive oxygen species, but independent of sirtuin1

forrás: http://www.ncbi.nlm.nih.gov/pubmed/22211760


2014-10-28 13:38:15


The currently available chemotherapeutic regimens against gastric cancer are not very effective, leading to high recurrence and poor survival. Resveratrol is a naturally occurring polyphenol with potent apoptosis-inducing activity. However, the mechanism underlying its actions remains unknown. In the present study, human gastric adenocarcinoma SGC7901 cells were treated with resveratrol (0, 25, 50, 100 and 200 μmol/L) for 48 h, and cellular apoptosis DNA damage were determined. In certain experiments, cells were incubated with superoxide dismutase (100 U/mL), catalase (300 U/mL) or sirtinol (10 μmol/L) to determine the role of reactive oxygen species (ROS) and sirtuin1 in resveratrol-induced cellular apoptosis. Treatment with resveratrol (50-200 μmol/L) for 48 h significantly induced apoptosis and DNA damage in human gastric cancer SGC7901 cells. This was due to the increased generation of ROS following resveratrol treatment because incubation of cells with superoxide dismutase (100 U/mL) or catalase (300 U/mL) attenuated resveratrol-induced cellular apoptosis. Interestingly, treatment with resveratrol (25-200 μmol/L) did not affect the level and activity of sirtuin1, whereas the sirtuin1 inhibitor sirtinol (10 μmol/L) significantly reduced sirtuin1 activity. Furthermore, treatment with sirtinol (10 μmol/L) did not have any effect on apoptosis induced by resveratrol. These data provide evidence that resveratrol induces apoptosis via ROS, but independent of sirtuin1, in the human gastric cancer cell line SGC7901.


A low dose of dietary resveratrol partially mimics caloric restriction and retards aging parameters in mice

forrás: http://www.ncbi.nlm.nih.gov/pubmed/18523577


2014-10-28 13:29:37


Resveratrol in high doses has been shown to extend lifespan in some studies in invertebrates and to prevent early mortality in mice fed a high-fat diet. We fed mice from middle age (14-months) to old age (30-months) either a control diet, a low dose of resveratrol (4.9 mg kg(-1) day(-1)), or a calorie restricted (CR) diet and examined genome-wide transcriptional profiles. We report a striking transcriptional overlap of CR and resveratrol in heart, skeletal muscle and brain. Both dietary interventions inhibit gene expression profiles associated with cardiac and skeletal muscle aging, and prevent age-related cardiac dysfunction. Dietary resveratrol also mimics the effects of CR in insulin mediated glucose uptake in muscle. Gene expression profiling suggests that both CR and resveratrol may retard some aspects of aging through alterations in chromatin structure and transcription. Resveratrol, at doses that can be readily achieved in humans, fulfills the definition of a dietary compound that mimics some aspects of CR.


Clinical experience with the use of Flavin7®, Pilot Study

forrás: http://www.researchgate.net/publication/242586746_Clinical_experience_with_the_use_of_Flavin7_Pilot_Study


2014-02-24 11:05:48


Aims:

Prevention of malignant diseases, improvement of the quality of life of patients under cancer treatment regimens, care and rehabilitation. Observation of clinical status, changes in the course of the disease with emphasis on the quality of life during the use of the high bioflavonoid content Flavin7® dietary supplement.

Methods:

Dietary supplement, Flavin7® was added to standard treatments, randomized,multi-center, controlled clinical trial on 295 patients in 33 centers.

Results:

Flavin7® is a dietary supplement containing bioflavonoid, resveratrol, anthocyanides, vitamins, minerals and trace-minerals. Its oxidative radical scavenging activity is a function of concentration and time, and exceeds the effectiveness of similar products on the market. In this study, the treating physicians found that Flavin7® inhibits the growth of tumours, reduces or alleviates the side effects of chemotherapy and radiation therapy. Flavin7® displays hepatoprotective properties and protects against cardio toxicity. Reduces pain caused by bone metastases. The treating physicians observed improved quality of life and symptoms of depression.

Conclusions:

The review of international scientific literature and epidemiology data suggest that the role of bioflavonoids is more accepted in prevention. Presently, the scientifically proven treatment methods are not successful to reasonably improve quality of life, survival figures, complete and partial remission. Flavin7® given as a dietary supplement markedly improved the quality of life and the ratio of complete and partial remission of our patients. Our clinical observations suggest that the introduction of bioflavonoid supplements can effectively reduce the cardiovascular and cancer morbidity, mortality and chemo-prevention could become more successful. We strongly suggest further clinical research that meets the expectations and international standards of the society of oncologists. In the challenging field of oncology there is a place for complementary modalities of chemo-prevention and symptom-control.


Az emberi monocita szöveti faktor indukció gátlása vörösbor fenolok és a resveratrol szintetikus származékainak révén

forrás: http://www.ncbi.nlm.nih.gov/pubmed/16507316


2014-10-28 13:42:48


A szív- és érrendszeri megbetegedések táplálék kiegészítők által történő megelőzésének népszerűsége szerte a világon növekszik. Több epidemiológiai tanulmányban is úgy látták, hogy a mérsékelt alkoholfogyasztás, különösen a vörösboré, csökkenti a koszorúeres szívbetegségek [CHD – coronary heart disease] esetében a halálozási arányt. A CHD-vel kapcsolatos krónikus gyulladás és érelmeszesedés [atherosclerosis] a szöveti faktor [TF – tissue factor, a subendotheális szövetekben található fehérje] rendellenes éren belüli [intravaszkuláris] kifejeződésében tetőződik, amely vérrögképződést indít el, ami trombózishoz, a szívinfarktus fő okához vezet. Korábban már kimutattuk, hogy két vörösbor fenol, a resveratrol és a quercetin elnyomja a TF indukciót az ér belső falát alkotó vékony sejtekben, az endotheliális sejtekben. A jelen kutatásban hét olyan resveratrol származék hatékonyságát vizsgáltuk a TF indukció gátlását tekintve a periferiális vér mononukleáris  sejteknél [PBMC – peripheral blood mononuclear cell],  amelyek in vitro hatásosak voltak a rákos sejtnövekedés szabályozásában sokkal alacsonyabb koncentrációkban, mint az eredeti resveratrol vegyületben. Vizsgáltuk a resveratrol és a quercetin lehetséges szinergisztikus [együttható] hatásait a többi fő vörösbor fenolokkal együtt a lipopolysaccharid [zsír polisaccharid] indukálta TF kifejeződések gátlásában az emberi PBMC-kben. Azt találtuk, hogy számos resveratrol származék 2-10-szer hatékonyabb volt, mint a resveratrol a TF indukció gátlásában. Kutatásunk nem talált bizonyítékot szinergizmusra [amikor két együttható kombinációja erősebb, mint az együtthatók egyenként] a vörös bor poliphenolok között. Ezek az adatok azt sugallják, hogy a resveratrol szerkezeti átalakításai hatásosak lehetnek olyan hatékony trombózis ellenes hatóanyagok előállításában, amelyek gyógyászati potenciállal rendelkeznek a szív- és érrendszeri egészség javításában és a CHD megelőzésében. A főbb vörös bor fenolok közül a quercetin tűnik a TF indukció döntő elnyomójának.


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