Oligosaccharide and peptidoglycan of Ganoderma lucidum activate the immune response in human mononuclear cells.

forr√°s: http://www.ncbi.nlm.nih.gov/pubmed/22364151


2014-02-18 10:06:55


The acid-hydrolyzed fragments of Ganoderma lucidum polysaccharides (GLPS) obtained by Smith degradation were separated by size-exclusion chromatography into two major water-soluble fractions: peptidoglycans (GLPS-SF1) and oligosaccharides (GLPS-SF2). Both fractions induced CD69 in human peripheral blood mononuclear cells (hPB-MNCs), and they displayed distinct immunomodulating properties. GLPS-SF1, with a molecular weight of around 20 kDa, were heterogeneous peptidoglycans composed of glucose/mannose (4:1) that exhibited biological activities with Th1 cytokines IL-12, IL-2, TNF-őĪ, and IFN-ő≥ in hPB-MNCs and stimulated macrophage cytokine expression via Toll-like receptor 4 (TLR4) signaling. For GLPS-SF2, with a molecular weight of around several kilodaltons, its sugar sequence was elucidated by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy as [-őĪ-1,4-Glc-(ő≤-1,4-GlcA)(3)-](n). This oligosaccharide displayed specific immune property with low monocyte induction, greatly stimulated cell activation and proliferation of NK and T cells. This oligosaccharide isolated from G. lucidumpolysaccharides with internal glucuronic acids/glucose repeat unit in a 3:1 ratio may be responsible for the active stimulation of NK and T cells.


Probing Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (higher Basidiomycetes): a bitter mushroom with amazing health benefits.

forr√°s: http://www.ncbi.nlm.nih.gov/pubmed/23557365


2014-02-18 10:03:26


Ganoderma lucidum (Lingzhi or Reishi) is known as a bitter mushroom with remarkable health benefits. The active constituents found in mushrooms include polysaccharides, dietary fibers, oligosaccharides, triterpenoids, peptides and proteins, alcohols and phenols, mineral elements (such as zinc, copper, iodine, selenium, and iron), vitamins, and amino acids. The bioactive components found in the G. lucidum mushroom have numerous health properties to treat diseased conditions such as hepatopathy, chronic hepatitis, nephritis, hypertension, hyperlipemia, arthritis, neurasthenia, insomnia, bronchitis, asthma, gastric ulcers, atherosclerosis, leukopenia, diabetes, anorexia, and cancer. In spite of the voluminous literature available, G. lucidum is used mostly as an immune enhancer and a health supplement, not therapeutically. This review discusses the therapeutic potential of G. luidum to attract the scientific community to consider its therapeutic application where it can be worth pursuing.


A Ganoderma lucidum oligoszacharid és peptid-glikánja aktiválja az immunválaszt az emberi mononukleáris sejtekben.

forr√°s: http://www.ncbi.nlm.nih.gov/pubmed/22364151


2014-02-18 10:06:55


A Ganoderma lucidum poliszacaharidok savval aktiv√°lt t√∂red√©kei/frakci√≥i (GLPS) √©s amelyeket Smith-degrad√°ci√≥val kaptunk, m√©ret-kiz√°r√°sos kromatogr√°fisan szepar√°ltunk k√©t fŇĎ, v√≠zben oldhat√≥ frakci√≥ra: peptid-glik√°nokra (GLPS-SF1) √©s oligoszacharidokra (GLPS-SF2). Mindk√©t frakci√≥ CD69-et v√°ltott ki az emberi perif√©ri√°s v√©r mononukle√°ris sejtjeiben (hPB-MNCs), √©s ezek k√ľl√∂nb√∂zŇĎ immunmodul√°l√≥ tulajdons√°gokat jeleztek. A GLPS-SF1, melynek molekulas√ļlya megk√∂zel√≠tŇĎleg 20 kDa heterog√©n peptid-glik√°nok voltak, melyek gl√ľk√≥zb√≥l/mann√≥zb√≥l (4:1) tevŇĎdtek √∂ssze, √©s amelyek biol√≥giai aktivit√°st mutattak ki a Th1 citokinekkel IL-12, IL-2, TNF-őĪ, √©s az IFN-ő≥ in hPB-MNCs-ben, valamint stimul√°lt√°k a makrof√°g (fal√≥sejtes) citokin expresszi√≥t a Toll-szerŇĪ receptoron kereszt√ľl 4 (TLR4) jelz√©s √ļtj√°n. A GLPS-SF2 eset√©ben t√∂bb kilodaltonos molekulas√ļlyn√°l, annak cukor szekvenci√°ja t√∂meg spektrometri√°san (MS) volt megvil√°gos√≠tva, valamint sejtm√°gneses rezonanci√°s (NMR) spektroszk√≥pi√°val [-őĪ-1,4-Glc-(ő≤-1,4-GlcA)(3)-](n). Ez az oligoszacharid, mely specifikus immun tulajdons√°gokat mutatott ki alacsony monocita indukci√≥val, erŇĎsen stimul√°lta a sejtaktiv√°ci√≥t √©s sejtszaporod√°st az NK √©s T sejtekben. Ez az oligoszacharid, melyet G. lucidum polysacharidokb√≥l izol√°lt√°k belsŇĎ gl√ľk√≥zsavakkal/ gl√ľk√≥z ism√©tlŇĎ egys√©ggel 3:1ar√°nyban, lehet felelŇĎs az NK √©s T sejtek akt√≠v stimul√°l√°s√°√©rt.


A Lingzhi vagy Reishi gy√≥gygomb√°k Ganoderma lucidum tesztje (magasabb Bazidiomycetes): egy keserŇĪ gombaf√©le, melynek csod√°latosan j√≥t√©kony hat√°sa van az eg√©szs√©gre.

forr√°s: http://www.ncbi.nlm.nih.gov/pubmed/23557365


2014-02-18 10:03:26


A Ganoderma lucidum (Lingzhi or Reishi) arr√≥l ismert, hogy egy keserŇĪ gombaf√©le, amelynek csod√°latosan j√≥t√©kony hat√°sa van az eg√©szs√©gre. Azok a hat√≥anyagok, amelyeket ez a gomba tartalmaz, magukban foglalj√°k a poliszacharidokat, √©trendi rostokat, oligoszacharidokat, triterpenoidokat, peptideket √©s feh√©rj√©ket, alkoholokat √©s fenolokat, √°sv√°nyi elemeket (mint pl. a cink, r√©z, j√≥d, szel√©nium √©s vas), vitaminokat √©s aminosavakat. A G. lucidum gomb√°ban tal√°lhat√≥ bioakt√≠v komponenseknek sz√°mos, eg√©szs√©gre j√≥t√©kony hat√°sa van, √≠gy kezelni lehet vele olyan betegs√©geket, mint a m√°jbetegs√©g, kr√≥nikus m√°jgyullad√°s, vesegyullad√°s, magas v√©rnyom√°s, hiperglik√©mia, arthritis, neuraszt√©nia, √°lmatlans√°g, l√©gcsŇĎhurut, asztma, gyomorfek√©lyek, agy√©relmeszesed√©s, leukop√©nia, cukorbetegs√©g, anorexia √©s r√°k. A t√∂bb k√∂tetnyi el√©rhetŇĎ szakirodalom ellen√©re a G. lucidum gomb√°t immunerŇĎs√≠tŇĎk√©nt haszn√°lj√°k leggyakrabban √©s t√°pl√°l√©k-kieg√©sz√≠tŇĎk√©nt, nem ter√°piaszerŇĪen. Ez a tanulm√°ny a G. lucidum lehets√©ges gy√≥gy√°szati lehetŇĎs√©geit vizsg√°lja, hogy fel lehessen h√≠vni a tud√≥s k√∂z√∂ss√©gek figyelm√©t annak m√©rlegel√©s√©re, hogyan lehetne alkalmazni a gomb√°t gy√≥gy√°szatilag, ahol csak √©rdemes √©s lehets√©ges.


Oligozaharidele Ňüi peptidoglicanii din Ganoderma lucidum activeazńÉ rńÉspunsul imun √ģn celulele mononucleare umane.

forr√°s: http://www.ncbi.nlm.nih.gov/pubmed/22364151


2014-02-18 10:06:55


Fragmentele hidrolizate cu acid ale polizaharidelor din Ganoderma Lucidum (GLPS), obŇ£inute prin degradare Smith, au fost separate, prin cromatografie de excludere a mńÉrimii, √ģn douńÉ fracŇ£ii majore solubile √ģn apńÉ: peptidoglicani (GLPS-SF1) Ňüi oligozaharide (GLPS-SF2). Ambele fracŇ£iuni au indus CD69 √ģn celulele umane mononucleare din s√Ęngele periferic (hPB-CMN), Ňüi au demonstrat proprietńÉŇ£i distincte imunomodulatoare. GLPS-SF1, cu o masńÉ molecularńÉ de aproximativ 20 kDa, au fost peptidoglicani heterogeni compuŇüi din glucozńÉ/manozńÉ (4:1), care au desfńÉŇüurat activitńÉŇ£i biologice cu citochinele Thl IL-12, IL-2, TNF-őĪ, Ňüi IFN-ő≥ √ģn hPB-CMN Ňüi au stimulat expresia citochinei macrofage prin intermediul semnalizńÉrii receptorilor Toll-like 4 (TLR4). √én cazul GLPS-SF2, cu o masńÉ molecularńÉ de aproximativ c√ĘŇ£iva kilodaltoni, secvenŇ£a sa de zahńÉr a fost elucidatńÉ prin spectrometrie de masńÉ (MS) Ňüi spectroscopie prin rezonanŇ£ńÉ magneticńÉ nuclearńÉ (RMN) ca fiind: [-őĪ -1,4-Glc-(ő≤-1,4-GlcA)(3)-](n). AceastńÉ oligozaharidńÉ a demonstrat proprietńÉŇ£i imune specifice cu inducŇ£ie de monocite scńÉzutńÉ, stimul√Ęnd √ģn mare mńÉsurńÉ activarea celulelor Ňüi proliferarea celulelor NK Ňüi T. AceastńÉ oligozaharidńÉ, izolatńÉ  de polizaharidele din G. lucidum cu  o fracŇ£ie  internńÉ repetatńÉ de acizi glucuronici / glucozńÉ √ģn-un raport de 3:1, poate fi responsabilńÉ pentru stimularea activńÉ a celulelor NK Ňüi T.


QR-CODE

Download image: Right click, "Image save as..."